Acetic Acid Assay Kit (AK; rapid manual format)

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages, and is compatible with both manual and microplate formats. Includes 72 assays (manual) / 720 assays (microplate).
SKU: 5WZZZZZ2005
$236.00

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.Includes 72 assays (manual) / 720 assays (microplate).

 

Advantages

• Improved assay format (only two absorbance readings required)

• All reagents stable for > 2 years after preparation

• PVP incorporated to prevent tannin inhibition

• Very rapid reaction (~ 4 min)

• Mega-Calc™ software tool for hassle-free raw data processing

• Very competitive price (cost per test)

• Suitable for manual and microplate formats

Principle:

(acetate kinase)

(1) Acetic acid + ATP → acetyl-phosphate + ADP



(phosphotransacetylase)

(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi



(pyruvate kinase)

(3) ADP + PEP → ATP + pyruvate



(D-lactate dehydrogenase)

(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

 

Kit size: 72 assays (manual) / 720 (microplate)

Method: Spectrophotometric at 340 nm

Reaction time: ~ 4 min

Detection limit: 0.254 mg/L

 

DATA BOOKLET

SDS

MEGA-CALC

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.Includes 72 assays (manual) / 720 assays (microplate).

 

Advantages

• Improved assay format (only two absorbance readings required)

• All reagents stable for > 2 years after preparation

• PVP incorporated to prevent tannin inhibition

• Very rapid reaction (~ 4 min)

• Mega-Calc™ software tool for hassle-free raw data processing

• Very competitive price (cost per test)

• Suitable for manual and microplate formats

Principle:

(acetate kinase)

(1) Acetic acid + ATP → acetyl-phosphate + ADP



(phosphotransacetylase)

(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi



(pyruvate kinase)

(3) ADP + PEP → ATP + pyruvate



(D-lactate dehydrogenase)

(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

 

Kit size: 72 assays (manual) / 720 (microplate)

Method: Spectrophotometric at 340 nm

Reaction time: ~ 4 min

Detection limit: 0.254 mg/L

 

DATA BOOKLET

SDS

MEGA-CALC

Resource Guide
2017 Distilling Resource Catalog