This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.Includes 72 assays (manual) / 720 assays (microplate).

Compatible with MegaQuant™  Wave Spectrophotometer 

Advantages

• Improved assay format (only two absorbance readings required)

• All reagents stable for > 2 years after preparation

• PVP incorporated to prevent tannin inhibition

• Very rapid reaction (~ 4 min)

• Mega-Calc™ software tool for hassle-free raw data processing

• Very competitive price (cost per test)

• Suitable for manual and microplate formats

Principle:


(acetate kinase)

(1) Acetic acid + ATP → acetyl-phosphate + ADP



(phosphotransacetylase)

(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi



(pyruvate kinase)

(3) ADP + PEP → ATP + pyruvate



(D-lactate dehydrogenase)

(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size: 72 assays (manual) / 720 (microplate)

Method: Spectrophotometric at 340 nm

Reaction time: ~ 4 min

Detection limit: 0.254 mg/L

DATA BOOKLET

SDS

MEGA-CALC

Megazyme Knowledge Base