L-Asparagine/L-Glutamine/ Ammonia Assay Kit

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).
SKU: WZZZ2017
$250.00

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).

Compatible with MegaQuant™  Wave Spectrophotometer

Advantages

• Very rapid reaction due to use of uninhibited glutamate dehydrogenase

• All enzymes supplied as stabilised suspensions

• Only kit available

• Very cost effective

• All reagents stable for > 2 years after preparation

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

• Mega-Calc™ software tool for hassle-free raw data processing

• Standard included

 

Principle:


(glutaminase)

(1) L-Glutamine + H2O → L-glutamate + NH4+



(microbial glutamate dehydrogenase)

(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O



(asparaginase)

(3) L-Asparagine + H2O → L-aspartate + NH4+

 

Kit size: 50 assays of each

Method: Spectrophotometric at 340 nm

Reaction time: ~ 20 min

Detection limit: 0.50 mg/L (L-asparagine) 0.54 mg/L (L-glutamine) 0.06 mg/L (ammonia)

 

DATA BOOKLET 

SDS 

MEGA-CALC 

Megazyme Knowledge Base 

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).

Compatible with MegaQuant™  Wave Spectrophotometer

Advantages

• Very rapid reaction due to use of uninhibited glutamate dehydrogenase

• All enzymes supplied as stabilised suspensions

• Only kit available

• Very cost effective

• All reagents stable for > 2 years after preparation

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

• Mega-Calc™ software tool for hassle-free raw data processing

• Standard included

 

Principle:


(glutaminase)

(1) L-Glutamine + H2O → L-glutamate + NH4+



(microbial glutamate dehydrogenase)

(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O



(asparaginase)

(3) L-Asparagine + H2O → L-aspartate + NH4+

 

Kit size: 50 assays of each

Method: Spectrophotometric at 340 nm

Reaction time: ~ 20 min

Detection limit: 0.50 mg/L (L-asparagine) 0.54 mg/L (L-glutamine) 0.06 mg/L (ammonia)

 

DATA BOOKLET 

SDS 

MEGA-CALC 

Megazyme Knowledge Base 

Resource Guide